The cell and tissue engineering facility of the IFM provides support and advices for the use of human pluripotent stem cells to study neurodevelopment and neurological disorders. It provides access to a dedicated culture room, different induced pluripotent stem cell lines (hiPS, wild type and reporter cell lines) as well as technical supervision.

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Activity

• Amplification, banking and quality control of hiPS cell lines
• iPS differentiation in : 1) neural progenitors [motorneurones (Maury et al, 2015, Mouilleau, Vaslin et al 2021) ; spinal interneurons (Maury et al, 2015) ; cortical neurons (Gribaudo et al., 2019) ; brainstem neurons (Maury et al, 2015)…] and 2) spinal organoids (Duval et al, 2019)
• Standardized and ready to use banks of neuronal progenitors
• Neuronal culture
• Training and formation : culture and differentiation of hiPSC in neural progenitors

Materials

The cell and tissue engineering facility dispose of two culture rooms (L2) provided with:

• 3 culture hood
• 5 incubators, including one allowing hypoxia experiments
• -150°C and liquid nitrogen tanks
• 2 inverted microscopes   
• 1 stereomicroscope

Staff

• Simona GRIBAUDO, Research Engineer, SU, in charge
• Marion RUSSEAU, Technician, INSERM

Stéphane NEDELEC, CRCN INSERM, scientific advisor