Pleiotropy of morphogens
Our research team focuses on the molecular mechanisms underlying the multiple roles of morphogens during the nervous system development.
During embryonic development, the formation of the brain by the generation, differentiation and finally the precise connection of new neurons is controlled by many molecular factors. Amongst these factors, extracellular secreted molecules, called morphogens, instruct the shape of the nervous system. During neuronal differentiation, cells change from a proliferative state to acquire cell identity, to perform migration and finally to grow axons and connect to other cell types. Thus, cells need to change their responses to their environment to achieve the correct behavior. Our aim is to answer to the following question: how do morphogenic signals control distinct responses during neural development? Our research addresses fundamental questions on how a single (and then multiple) morphogenic signal(s) act at different steps of neuronal development to induce these different responses. We use the murine and human developing cerebral cortex as a model.
- In vitro primary cell cultures of cortical progenitors and neurons
- Microfluidic chambers
- In utero electroporation
- 3D imaging of embryos
- Molecular Biology (RNA-seq, ATAC-seq)
We are involved in teaching programs such as:
- Sorbonne Université – Master Biologie Moléculaire et Cellulaire – «Communication et Signalisation Cellulaire»
- Ecole Normale Supérieure Paris-Saclay – M2 FESup SV-BGB Préparation à l’agrégation de Biochimie – «Neurobiologie cellulaire»
- Sorbonne Université – Master Biologie Intégrative et Physiologie – «Guidage axonal»
Our research team is supported by INSERM, The ATIP-Avenir program, the Fyssen Foundation and the Brain & Behavior Research Foundation.
Julien Ferent, PhD, CRCN (INSERM), Principal Investigator
Sara Douceau, PhD, Post-doctoral fellow
Tanya Deutsch Guerrero, PhD student, Sorbonne University
Hanna Kavaliova, Undergrade, Alumni
André Heilser, Undergrade, Alumni
Etienne Poidomani, Undergrade, Alumni
Most Recent Publications
Establishing Hedgehog Gradients during Neural Development
Douceau S, Deutsch Guerrero T, Ferent J.
Cells. 2023 Jan 5;12(2):225.
Heterozygous Dcc Mutant Mice Have a Subtle Locomotor Phenotype
Thiry L, Lemaire C, Rastqar A, Lemieux M, Peng J, Ferent J, Roussel M, Beaumont E, P Fawcett J, M Brownstone R, Charron F, Bretzner F.
eNeuro. 2022 Mar 8;9(2):ENEURO.0216-18.2021.
Extracellular Control of Radial Glia Proliferation and Scaffolding During Cortical Development and Pathology
Ferent J, Zaidi D, Francis F
Front Cell Dev Biol. (2020) 8: 578341
The Ciliary Protein Arl13b Functions Outside of the Primary Cilium in Shh-Mediated Axon Guidance
Ferent J, Constable S, Gigante ED, Yam PT, Mariani LE, Legué E, Liem KF Jr, Caspary T, Charron F
Boc Acts via Numb as a Shh-Dependent EndocyticPlatform for Ptch1 Internalization and Shh-MediatedAxon Guidance
Ferent J, Giguère F, Jolicoeur C, Morin S, Michaud JF, Makihara S, Yam PT, Cayouette M, Charron F
Neuron. 2019 Jun 19;102(6):1157-1171.e5
The Shh receptor Boc is important for myelin formation and repair
Zakaria M, Ferent J, Hristovska I, Laouarem Y, Zahaf A, Kassoussi A, Mayeur ME, Pascual O, Charron F, Traiffort E
Development. 2019 May 2;146(9):dev172502
Polarized Dock Activity Drives Shh-Mediated Axon Guidance
Makihara S, Morin S, Ferent J, Côté JF, Yam PT, Charron F.
Dev Cell. 2018 Aug 20;46(4):410-425.e7.
Hedgehog: A Key Signaling in the Development of the Oligodendrocyte Lineage
Traiffort E, Zakaria M, Laouarem Y, Ferent J.
J Dev Biol. 2016 Sep 8;4(3):28.
Loss of Dcc in the spinal cord is sufficient to cause a deficit in lateralized motor control and the switch to a hopping gait
Peng J, Ferent J, Li Q, Liu M, Da Silva RV, Zeilhofer HU, Kania A, Zhang Y, Charron F.
Dev Dyn. 2018 Apr;247(4):620-629.
Cellules souches neurales et signalisation Notch
Traiffort E, Ferent J.
Med Sci (Paris). 2015 Dec;31(12):1115-25.